uniCAS
Abstract
Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently discovered and highly promising CRISPR/Cas9 system. The regulation is based on the RNA-guided Cas9 protein, which allows targeting of specific DNA sequences. Our toolkit comprises not only a standardized Cas9 protein, but also different effector domains for efficient gene activation or repression. We further engineered a modular RNA plasmid for easy implementation of RNA guide sequences. As an additional feature, we established an innovative screening method for assessing the functionality of our uniCAS fusion proteins. Single genes and even whole genetic networks can be modified using our uniCAS toolkit. We think that our toolbox of standardized parts of the CRISPR/Cas9 system offers broad application in research fields such as tissue engineering, stem cell reprogramming and fundamental research.
Wiki
https://2013.igem.org/Team:Freiburg
Achievements
- Best Foundational Advance Project, Overgrad
- Gold Medal
- 1st Runner up - Regional Jamboree Europe
- Best New BioBrick Part or Device, Engineered, Overgrad - Regional Jamboree Europe
Meet The Team
Primary PI
Student Members
- Alica Emhardt
- Alina Rudorf
- Anne Müller
- Christoph Gaebelein
- Fenja Gawlas
- Lisa Schaefer
- Lisa Schmunk
- Manuel Scheidmann
- Maximilian Stammnitz
- Michael Agne
- Nadine Gillich
- Natalie Louis
- Patrick Gonschorek
- Philipp Schwenk
- Philipp Warmer
- Stefan Krämer
- Thomas Juretschke
Advisors
Instructors
- Adrian Fischer
- Beate Kaufmann
- Hannah Wagner
- Maximilian Hörner
- Patrick Schindler