DiaCHIP - Enlightening Diagnostics
Abstract
In modern medicine, fast detection and differentiation of diseases is a crucial and fundamental task. Typical ELISA-based assays are time-consuming and expensive. We propose an advanced procedure for the simultaneous detection of various diseases in a fast and inexpensive manner, the DiaCHIP. Our approach is based on the interaction of antibodies with their respective antigens. Different antigens are immobilized on a protein array generated by cell-free protein expression, using the corresponding DNA array as a template. Placed in a microfluidic chamber, the protein array is incubated with a patient’s blood sample. The interaction between an antibody in the sample and the corresponding immobilized antigen results in a local change of the optical thickness of the surface. This change can be detected using a label-free and real-time measurement technology called iRIf (imaging Reflectometric Interference). Offering simultaneous screening for several diseases, our DiaCHIP has strong potential to improve future diagnostics.
Wiki
https://2015.igem.org/Team:Freiburg
Achievements
- Gold Medal
- Best Health and Medicine Project, Overgrad - Nomination
- Best Presentation, Overgrad - Nomination
- Best Wiki, Overgrad - Nomination
- Best Innovation in Medicine, Overgrad - Nomination
Meet The Team
Primary PI
Secondary PI
Student Members
- Anne Seifert
- Benjamin Werle
- Daniel Kokoket
- Julia Donauer
- Julia Zbiegly
- Julian Bender
- Julian Eble
- Julika Neumann
- Katharina Moos
- Lara Stühn
- Luisa Keilholz
- Maurizio Camagna
- Nathalie Wössner
- Rabea Jesser
- Ramona Emig
- Ricardo Oude Weernink
- Sabine Bognar
- Sabrina Fischer
- Simon Nicklaus
Advisors
- Christin Rath
- Ilona Blank
- Juergen Burger
- Norman Kilb
- Patrick Gonschorek
- Philipp Andreas Meyer
- Philipp Schwenk
- Stefan Krämer
- Tobias Herz