DiaCHIP - Enlightening Diagnostics

Abstract

In modern medicine, fast detection and differentiation of diseases is a crucial and fundamental task. Typical ELISA-based assays are time-consuming and expensive. We propose an advanced procedure for the simultaneous detection of various diseases in a fast and inexpensive manner, the DiaCHIP. Our approach is based on the interaction of antibodies with their respective antigens. Different antigens are immobilized on a protein array generated by cell-free protein expression, using the corresponding DNA array as a template. Placed in a microfluidic chamber, the protein array is incubated with a patient’s blood sample. The interaction between an antibody in the sample and the corresponding immobilized antigen results in a local change of the optical thickness of the surface. This change can be detected using a label-free and real-time measurement technology called iRIf (imaging Reflectometric Interference). Offering simultaneous screening for several diseases, our DiaCHIP has strong potential to improve future diagnostics.

Wiki

https://2015.igem.org/Team:Freiburg¹

Achievements

• Gold Medal
• Best Health and Medicine Project, Overgrad - Nomination
• Best Presentation, Overgrad - Nomination
• Best Wiki, Overgrad - Nomination
• Best Innovation in Medicine, Overgrad - Nomination

Meet The Team

Primary PI

• Dr. Maximillian Ulbrich²

Secondary PI

• Dr. Nicole Gensch³
• Dr. Günter Roth⁴

Student Members 

• Anne Seifert⁵
• Benjamin Werle
• Daniel Kokoket⁶
• Julia Donauer⁷
• Julia Zbiegly⁸
• Julian Bender⁹
• Julian Eble¹⁰
• Julika Neumann¹¹
• Katharina Moos
• Lara Stühn¹²
• Luisa Keilholz¹³
• Maurizio Camagna¹⁴
• Nathalie Wössner¹⁵
• Rabea Jesser¹⁶
• Ramona Emig¹⁷
• Ricardo Oude Weernink¹⁸
• Sabine Bognar¹⁹
• Sabrina Fischer²⁰
• Simon Nicklaus²¹

Advisors

• Christin Rath²²
• Ilona Blank²³
• Juergen Burger
• Norman Kilb²⁴
• Patrick Gonschorek²⁵
• Philipp Andreas Meyer²⁶
• Philipp Schwenk²⁷
• Stefan Krämer²⁸
• Tobias Herz²⁹